SInCRe - Rv0956

Tuberculist information

Gene name	purN
Protein function	Probable 5'-phosphoribosylglycinamide formyltransferase PurN (GART) (gar transformylase) (5'-phosphoribosylglycinamide transformylase)
Functional category(tuberculist)	intermediary metabolism and respiration
Gene location(kb)	1067.56
Molecular mass(da)	22383.7
External sites	TB Database TubercuList WebTB

Protein sequence

Number of amino acids : 215

	  		  	1    VQEPLRVPPSAPARLVVLASGTGSLLRSLLDAAVGDYPARVVAVGVDRECRAAEIAAEAS   60
			  	61   VPVFTVRLADHPSRDAWDVAITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPA  120
			  	121  LLPAFPGTHGVADALAYGVKVTGATVHLVDAGTDTGPILAQQPVPVLDGDDEETLHERIK  180
			  	181  VTERRLLVAAVAALATHGVTVVGRTATMGRKVTIG

Known structures in the PDB

	PDB ID	3DA8
	Total residue count	430
	Number of chains	2
	Method	X-RAY
	Resolution	1.3

	PDB ID	3DCJ
	Total residue count	430
	Number of chains	2
	Method	X-RAY
	Resolution	2.2

Number of amino acids : 215

Domains assigned based on sequence.

Mtb Structural proteome models

CHOPIN models

Profile-based domain assignment

Structural/Functional domain family	Pfam acc.no./SCOP ID	Domain Region
Formyl_trans_N	PF00551	13-191

Mtb Structural Proteome models

Model Number	1
Model name	Rv0956_3da8_A
Template	3DA8 APDB CREDO
Template coverage	3_215
Template Identity(%)	100.0
Model coverage(%)	98.6
Normalized DOPE score	-1.392

Model Number	2
Model name	Rv0956
Template	1JKX APDB CREDO
Template coverage	1_196
Template Identity(%)	35.1
Model coverage(%)	89.8
Normalized DOPE score	-0.861

Structure Models from Chopin

Model Number	1
Profile	c.65.1.1 - 3.40.50.170 Click for model details
zscore	50.2
Residue begin	13
Residue end	215

Binding pockets

STRING

Cytoscape Web will replace the contents of this div with protein-protein interaction network.

Protein interacting with Rv0956	Gene name	Confidence Score
Rv0772	purD	0.999
Rv0957	purH	0.999
Rv0772	purD	0.999
Rv0788	purQ	0.999
Rv0772	purD	0.999
Rv0957	purH	0.999
Rv0809	purM	0.998
Rv0809	purM	0.998
Rv3356c	folD	0.997
Rv3356c	folD	0.997
Rv0389	purT	0.997
Rv3275c	purE	0.996
Rv3276c	purK	0.995
Rv0803	purL	0.994
Rv0803	purL	0.994
Rv0808	purF	0.99
Rv0808	purF	0.99
Rv0787A	Rv0787A	0.986
Rv0777	purB	0.975
Rv0777	purB	0.975
Rv0780	purC	0.969
Rv1093	glyA1	0.964
Rv0070c	glyA2	0.96
Rv3396c	guaA	0.954
Rv3396c	guaA	0.954
Rv3396c	guaA	0.954
Rv1406	fmt	0.952
Rv1406	fmt	0.952
Rv2763c	dfrA	0.948
Rv0357c	purA	0.945
Rv2139	pyrD	0.943
Rv2754c	thyX	0.938
Rv2211c	gcvT	0.933
Rv2211c	gcvT	0.933
Rv2964	purU	0.927
Rv2964	purU	0.927
Rv1389	gmk	0.914
Rv2124c	metH	0.91
Rv2124c	metH	0.91
Rv2124c	metH	0.91
Rv2124c	metH	0.91
Rv2124c	metH	0.91
Rv3411c	guaB2	0.901
Rv3411c	guaB2	0.901

Potential ligand/drug binding sites

No binding sites identified

Similarity to known drug target from sensitive sequence analysis

No drug targets

List of small molecules tested from TIBLE

Data not available

Off-target activity - ligand based from TIBLE

Data not available

Small molecules involved in protein-protein complex from TIMBAL .Proteins similar to Mtb based on sequence analysis.

Data not available

STITCH interactions

Chemical Name	Confidence score	Molecular Weight
glycinamide ribonucleotidePubChem	0.982	286.176
tetrahydrofolatePubChem	0.969	445.429
10-formyltetrahydrofolatePubChem	0.969	473.439
magnesiumPubChem	0.966	24.305
5-methyltetrahydrofolatePubChem	0.958	459.456
H4FPubChem	0.945	445.429
formylglycinamide ribonucleotidePubChem	0.941	314.186
FGARPubChem	0.941	314.186
5'-phosphoribosyl-N-formylglycinamidePubChem	0.937	314.186
iodidePubChem	0.934	127.912
methenyltetrahydrofolatePubChem	0.9	456.432
5,10-methenyltetrahydrofolatePubChem	0.9	456.432
methyltetrahydrofolatePubChem	0.9	459.456
chloridePubChem	0.9	35.453
protiumPubChem	0.9	2.01576
hydroxyl radicalsPubChem	0.9	17.0073
H(+)PubChem	0.9	2.01588
2-mercaptoethanolPubChem	0.9	78.1334
deuteriumPubChem	0.9	2.01588
10-formyltetrahydropteroylglutamic acidPubChem	0.9	473.439
nitratePubChem	0.9	62.0049
NSC333308PubChem	0.9	286.176