SInCRe - Rv1326c

Tuberculist information

Gene name	glgB
Protein function	1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme)
Functional category(tuberculist)	intermediary metabolism and respiration
Gene location(kb)	1490.12
Molecular mass(da)	81729.5
External sites	TB Database TubercuList WebTB

Protein sequence

Number of amino acids : 731

	  		  	1    MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVG   60
			  	61   KDRFSLQHLDSGLFAVALPFVDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAE  120
			  	121  GRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSLIGEFNGWNGHEAPMRVLGP  180
			  	181  SGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD  240
			  	241  WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPF  300
			  	301  AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT  360
			  	361  PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD  420
			  	421  YSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGG  480
			  	481  LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT  540
			  	541  LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF  600
			  	601  SNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSANNVLSFMRYGSDGSVLACVF  660
			  	661  NFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL  720
			  	721  PPTSALWLTPA

Known structures in the PDB

	PDB ID	3K1D
	Total residue count	722
	Number of chains	1
	Method	X-RAY
	Resolution	2.33

Number of amino acids : 731

Domains assigned based on sequence.

Mtb Structural proteome models

CHOPIN models

Profile-based domain assignment

Structural/Functional domain family	Pfam acc.no./SCOP ID	Domain Region
CBM_48	PF02922	129-216
Alpha-amylase	PF00128	275-353
Alpha-amylase_C	PF02806	633-731

Mtb Structural Proteome models

Model Number	1
Model name	Rv1326c_3k1d_A
Template	3K1D APDB CREDO
Template coverage	10_731
Template Identity(%)	100.0
Model coverage(%)	98.6
Normalized DOPE score	-1.446

Model Number	2
Model name	Rv1326c
Template	1M7X APDB CREDO
Template coverage	117_725
Template Identity(%)	51.5
Model coverage(%)	83.2
Normalized DOPE score	-0.994

Structure Models from Chopin

Model Number	1
Profile	b.1.18.21 Click for model details
zscore	4.04
Residue begin	141
Residue end	216

Binding pockets

STRING

Cytoscape Web will replace the contents of this div with protein-protein interaction network.

Protein interacting with Rv1326c	Gene name	Confidence Score
Rv1213	glgC	0.999
Rv1328	glgP	0.999
Rv1328	glgP	0.999
Rv1213	glgC	0.999
Rv0127	mak	0.993
Rv1327c	glgE	0.987
Rv1327c	glgE	0.987
Rv1564c	treX	0.982
Rv1564c	treX	0.982
Rv3490	otsA	0.963
Rv1781c	malQ	0.954
Rv1563c	treY	0.93
Rv0946c	pgi	0.93
Rv1562c	treZ	0.913
Rv1562c	treZ	0.913
Rv0126	treS	0.902
Rv0126	treS	0.902
Rv3068c	pgmA	0.901
Rv3068c	pgmA	0.901
Rv3068c	pgmA	0.901
Rv3068c	pgmA	0.901

Potential ligand/drug binding sites

Protein Model	Pocket Number	Drug Binding Site	PMIN Score	Pvalue PMIN	PMAX Score	Pvalue PMAX
Rv1326c_3k1d_A	7	2v0z_C41_C_1328 PDB CREDO	0.602	0.177	0.545	0.005
Rv1326c_3k1d_A	7	3prs_RIT_A_1001 PDB CREDO	0.571	0.215	0.544	0.005
Rv1326c_3k1d_A	7	1tco_FK5_C_509 PDB CREDO	0.602	0.176	0.532	0.007
Rv1326c_3k1d_A	7	1fap_RAP_A_108 PDB CREDO	0.660	0.115	0.526	0.007
Rv1326c_3k1d_A	7	2vn1_FK5_A_501 PDB CREDO	0.689	0.089	0.520	0.008
Rv1326c_3k1d_A	7	3nxu_RIT_A_600 PDB CREDO	0.653	0.122	0.506	0.010
Rv1326c_3k1d_A	7	3nxu_RIT_B_600 PDB CREDO	0.652	0.122	0.505	0.010
Rv1326c_3k1d_A	7	2v0z_C41_O_1327 PDB CREDO	0.599	0.180	0.503	0.010

Similarity to known drug target from sensitive sequence analysis

No drug targets

List of small molecules tested from TIBLE

Data not available

Off-target activity - ligand based from TIBLE

Data not available

Small molecules involved in protein-protein complex from TIMBAL .Proteins similar to Mtb based on sequence analysis.

Data not available

STITCH interactions

Chemical Name	Confidence score	Molecular Weight
glycogenPubChem	0.989	666.578
ADP-glucosePubChem	0.963	589.342
ADP-D-GLUCOSEPubChem	0.9	589.342
adenosine diphosphatePubChem	0.9	427.201
alpha-glucanPubChem	0.836	504.437
galactosePubChem	0.776	180.156
alpha-D-glucosePubChem	0.738	198.171
beta-glucanPubChem	0.735	504.437
glycerolPubChem	0.709	92.0938
BE-01PubChem	0.704	283.957