SInCRe - Rv2447c

Tuberculist information

Gene name	folC
Protein function	Probable folylpolyglutamate synthase protein FolC (folylpoly-gamma-glutamate synthetase) (FPGS)
Functional category(tuberculist)	intermediary metabolism and respiration
Gene location(kb)	2746.14
Molecular mass(da)	50779.3
External sites	TB Database TubercuList WebTB

Protein sequence

Number of amino acids : 487

	  		  	1    MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSP   60
			  	61   QRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQY  120
			  	121  VATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGR  180
			  	181  WDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK  240
			  	241  VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAH  300
			  	301  NAVLALASVEAFFGAGAQRQLDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAS  360
			  	361  ALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDSVVVTHNGSPRALDVEALALA  420
			  	421  AGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART  480
			  	481  LFGRDPQ

Known structures in the PDB

	PDB ID	2VOR
	Total residue count	487
	Number of chains	1
	Method	X-RAY
	Resolution	2.3

	PDB ID	2VOS
	Total residue count	487
	Number of chains	1
	Method	X-RAY
	Resolution	2

Number of amino acids : 487

Domains assigned based on sequence.

Mtb Structural proteome models

CHOPIN models

Profile-based domain assignment

Structural/Functional domain family	Pfam acc.no./SCOP ID	Domain Region
Mur_ligase_M	PF08245	69-309
Mur_ligase_C	PF02875	335-418

Mtb Structural Proteome models

Model Number	1
Model name	Rv2447c_2vos_A
Template	2VOS APDB CREDO
Template coverage	24_489
Template Identity(%)	100.0
Model coverage(%)	95.5
Normalized DOPE score	-1.598

Model Number	2
Model name	Rv2447c
Template	1O5Z APDB CREDO
Template coverage	0_421
Template Identity(%)	33.9
Model coverage(%)	93.8
Normalized DOPE score	-0.368

Structure Models from Chopin

Model Number	1
Profile	c.59.1.2, c.72.2.2 - 3.40.1190.10, 3.90.190.20 Click for model details
zscore	66.33
Residue begin	22
Residue end	487

Model Number	2
Profile	c.72.2.2 Click for model details
zscore	53.1
Residue begin	25
Residue end	336

Model Number	3
Profile	c.59.1.2 Click for model details
zscore	23.71
Residue begin	335
Residue end	487

Model Number	4
Profile	3.90.190.20 Click for model details
zscore	12.31
Residue begin	335
Residue end	418

Binding pockets

STRING

Cytoscape Web will replace the contents of this div with protein-protein interaction network.

Protein interacting with Rv2447c	Gene name	Confidence Score
Rv3608c	folP1	0.996
Rv2448c	valS	0.994
Rv2448c	valS	0.994
Rv2448c	valS	0.994
Rv1207	folP2	0.993
Rv2763c	dfrA	0.993
Rv3455c	truA	0.979
Rv2764c	thyA	0.978
Rv3609c	folE	0.96
Rv3356c	folD	0.956
Rv3356c	folD	0.956
Rv2445c	ndkA	0.939
Rv3607c	folB	0.938
Rv3606c	folK	0.937
Rv1612	trpB	0.934
Rv0904c	accD3	0.92
Rv1408	rpe	0.915
Rv1611	trpC	0.906

Potential ligand/drug binding sites

No binding sites identified

Similarity to known drug target from sensitive sequence analysis

DrugBank ID	Drug Name
DB01015DrugBank	Sulfamethoxazole

List of small molecules tested from TIBLE

Data not available

Off-target activity - ligand based from TIBLE

Data not available

Small molecules involved in protein-protein complex from TIMBAL .Proteins similar to Mtb based on sequence analysis.

Data not available

STITCH interactions

Chemical Name	Confidence score	Molecular Weight
adenosine diphosphatePubChem	0.997	427.201
dihydrofolatePubChem	0.987	443.413
dihydropteroatePubChem	0.97	314.299
magnesiumPubChem	0.97	24.305
tetrahydrofolatePubChem	0.967	445.429
glycerolPubChem	0.951	92.0938
glutamatePubChem	0.943	147.129
AMP-PCPPubChem	0.923	505.208
methyltetrahydrofolatePubChem	0.921	459.456
5-methyltetrahydrofolatePubChem	0.919	459.456
ATP analogPubChem	0.919	505.208
NADPPubChem	0.913	744.413
H(+)PubChem	0.907	2.01588
deuteriumPubChem	0.906	2.01588
cobaltPubChem	0.9	117.866
phosphatePubChem	0.9	96.9872
protiumPubChem	0.9	2.01576
7,8-dihydropterin pyrophosphatePubChem	0.9	355.138
SeMetPubChem	0.9	196.106
selenomethioninePubChem	0.9	196.106
adenosine triphosphatePubChem	0.9	507.181
p-aminobenzoic acidPubChem	0.9	137.136
H4FPubChem	0.9	445.429
C09332PubChem	0.9	574.543
pyrophosphatePubChem	0.9	177.975
NADP(H)PubChem	0.9	745.421
folatePubChem	0.876	441.397
sulfatePubChem	0.774	96.0626
hydron sulfatePubChem	0.753	196.157
10-formyltetrahydrofolatePubChem	0.739	473.439
MS11PubChem	0.714	348.42