SInCRe - Rv0002

Tuberculist information

Gene name	dnaN
Protein function	DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase)
Functional category(tuberculist)	information pathways
Gene location(kb)	2.052
Molecular mass(da)	42113.1
External sites	TB Database TubercuList WebTB

Protein sequence

Number of amino acids : 402

	  		  	1    MDAATTRVGLTDLTFRLLRESFADAVSWVAKNLPARPAVPVLSGVLLTGSDNGLTISGFD   60
			  	61   YEVSAEAQVGAEIVSPGSVLVSGRLLSDITRALPNKPVDVHVEGNRVALTCGNARFSLPT  120
			  	121  MPVEDYPTLPTLPEETGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILGETVVLAA  180
			  	181  TDRFRLAVRELKWSASSPDIEAAVLVPAKTLAEAAKAGIGGSDVRLSLGTGPGVGKDGLL  240
			  	241  GISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVATMDVAELIEAIKLVALVADRGAQVRME  300
			  	301  FADGSVRLSAGADDVGRAEEDLVVDYAGEPLTIAFNPTYLTDGLSSLRSERVSFGFTTAG  360
			  	361  KPALLRPVSGDDRPVAGLNGNGPFPAVSTDYVYLLMPVRLPG

Known structures in the PDB

	PDB ID	3P16
	Total residue count	2448
	Number of chains	6
	Method	X-RAY
	Resolution	2.89

	PDB ID	3RB9
	Total residue count	790
	Number of chains	2
	Method	X-RAY
	Resolution	3

	PDB ID	4TR7
	Total residue count	804
	Number of chains	2
	Method	X-RAY
	Resolution	2.29

Number of amino acids : 402

Domains assigned based on sequence.

Mtb Structural proteome models

CHOPIN models

Profile-based domain assignment

Structural/Functional domain family	Pfam acc.no./SCOP ID	Domain Region
DNA_pol3_beta	PF00712	13-130
DNA_pol3_beta_2	PF02767	138-260
DNA_pol3_beta_3	PF02768	262-375

Mtb Structural Proteome models

Model Number	1
Model name	Rv0002
Template	2AVT APDB CREDO
Template coverage	2_376
Template Identity(%)	26.8
Model coverage(%)	96.0
Normalized DOPE score	-0.423

Structure Models from Chopin

Model Number	1
Profile	d.131.1.1 Click for model details
zscore	15.09
Residue begin	262
Residue end	375

Model Number	2
Profile	d.131.1.1 Click for model details
zscore	7.76
Residue begin	1
Residue end	118

Model Number	3
Profile	d.131.1.1 Click for model details
zscore	7.76
Residue begin	139
Residue end	256

Binding pockets

STRING

Cytoscape Web will replace the contents of this div with protein-protein interaction network.

Protein interacting with Rv0002	Gene name	Confidence Score
Rv0003	recF	0.999
Rv0001	dnaA	0.998
Rv0001	dnaA	0.998
Rv0005	gyrB	0.997
Rv0005	gyrB	0.997
Rv0005	gyrB	0.997
Rv0005	gyrB	0.997
Rv0006	gyrA	0.996
Rv0006	gyrA	0.996
Rv3924c	rpmH	0.988
Rv3721c	dnaZX	0.978
Rv3721c	dnaZX	0.978
Rv1617	pykA	0.975
Rv1617	pykA	0.975
Rv1547	dnaE1	0.957
Rv1547	dnaE1	0.957
Rv1547	dnaE1	0.957
Rv1547	dnaE1	0.957
Rv2445c	ndkA	0.955
Rv0321	dcd	0.936
Rv1420	uvrC	0.929
Rv1420	uvrC	0.929
Rv1420	uvrC	0.929
Rv1420	uvrC	0.929
Rv1021	Rv1021	0.922
Rv1633	uvrB	0.914
Rv1633	uvrB	0.914
Rv1633	uvrB	0.914
Rv1633	uvrB	0.914
Rv2344c	dgt	0.905
Rv2344c	dgt	0.905
Rv3056	dinP	0.903
Rv3056	dinP	0.903

Potential ligand/drug binding sites

No binding sites identified

Similarity to known drug target from sensitive sequence analysis

No drug targets

List of small molecules tested from TIBLE

Data not available

Off-target activity - ligand based from TIBLE

Data not available

Small molecules involved in protein-protein complex from TIMBAL .Proteins similar to Mtb based on sequence analysis.

Data not available

STITCH interactions

Chemical Name	Confidence score	Molecular Weight
thymidine triphosphatePubChem	0.9	482.168
deoxyadenosine triphosphatePubChem	0.9	491.182
deoxyguanosine triphosphatePubChem	0.9	507.181
deoxycytidine triphosphatePubChem	0.9	467.157