Rv2027c

Tuberculist information
Gene namedosT
Protein functionTwo component sensor histidine kinase DosT
Functional category(tuberculist)regulatory proteins
Gene location(kb)2272.79
Molecular mass(da)62136.5
External sites TB Database TubercuList WebTB
Protein sequence
Number of amino acids : 573
	  		  	1    VTHPDRANVNPGSPPLRETLSQLRLRELLLEVQDRIEQIVEGRDRLDGLIDAILAITSGL   60
			  	61   KLDATLRAIVHTAAELVDARYGALGVRGYDHRLVEFVYEGIDEETRHLIGSLPEGRGVLG  120
			  	121  ALIEEPKPIRLDDISRHPASVGFPLHHPPMRTFLGVPVRIRDEVFGNLYLTEKADGQPFS  180
			  	181  DDDEVLVQALAAAAGIAVDNARLFEESRTREAWIEATRDIGTQMLAGADPAMVFRLIAEE  240
			  	241  ALTLMAGAATLVAVPLDDEAPACEVDDLVIVEVAGEISPAVKQMTVAVSGTSIGGVFHDR  300
			  	301  TPRRFDRLDLAVDGPVEPGPALVLPLRAADTVAGVLVALRSADEQPFSDKQLDMMAAFAD  360
			  	361  QAALAWRLATAQRQMREVEILTDRDRIARDLHDHVIQRLFAVGLTLQGAAPRARVPAVRE  420
			  	421  SIYSSIDDLQEIIQEIRSAIFDLHAGPSRATGLRHRLDKVIDQLAIPALHTTVQYTGPLS  480
			  	481  VVDTVLANHAEAVLREAVSNAVRHANATSLAINVSVEDDVRVEVVDDGVGISGDITESGL  540
			  	541  RNLRQRADDAGGEFTVENMPTGGTLLRWSAPLR

Known structures in the PDB
PDB ID2VZW
Total residue count298
Number of chains2
MethodX-RAY
Resolution2.3
PDB ID3ZXQ
Total residue count248
Number of chains2
MethodX-RAY
Resolution1.9
1 58 115 172 229 286 343 400 457 514 571 Range: 61 to 198  |  Coverage: 23% GAF Range: 260 to 365  |  Coverage: 18% GAF_3 Range: 383 to 448  |  Coverage: 11% HisKA_3 Range: 486 to 573  |  Coverage: 15% HATPase_c Range: 60 to 205  |  Coverage: 25% 1 Range: 450 to 571  |  Coverage: 21% 2 Range: 441 to 570  |  Coverage: 22% 3 Range: 26 to 212  |  Coverage: 32% 4 Range: 486 to 572  |  Coverage: 15% 1 Range: 1 to 138  |  Coverage: 23% 2 Range: 229 to 366  |  Coverage: 23% 3
Number of amino acids : 573
Domains assigned based on sequence. Mtb Structural proteome models CHOPIN models
Profile-based domain assignment
Structural/Functional domain familyPfam acc.no./SCOP IDDomain Region
GAFPF0159061-198
GAF_3PF13492260-365
HisKA_3PF07730383-448
HATPase_cPF02518486-573
Mtb Structural Proteome models
Model Number1
Model nameRv2027c_2vzw_A
Template2VZW    APDBCREDO
Template coverage60_205
Template Identity(%)100.0
Model coverage(%)25.3
Normalized DOPE score-1.57

Model Number2
Model nameRv2027c.3
Template1YSR    APDBCREDO
Template coverage303_443
Template Identity(%)25.4
Model coverage(%)21.1
Normalized DOPE score-1.374

Model Number3
Model nameRv2027c.2
Template1ID0    APDBCREDO
Template coverage335_476
Template Identity(%)20.0
Model coverage(%)22.5
Normalized DOPE score-0.631

Model Number4
Model nameRv2027c
Template1MC0    APDBCREDO
Template coverage368_555
Template Identity(%)19.3
Model coverage(%)32.5
Normalized DOPE score-0.193

Structure Models from Chopin
Model Number1
Profiled.122.1.3 - 3.30.565.10
Click for model details
zscore14.16
Residue begin486
Residue end572
Model Number2
Profiled.110.2.1 - 3.30.450.40
Click for model details
zscore4.61
Residue begin1
Residue end138
Model Number3
Profiled.110.2.1 - 3.30.450.40
Click for model details
zscore4.61
Residue begin229
Residue end366
Binding pockets
STRING
Cytoscape Web will replace the contents of this div with protein-protein interaction network.
Protein interacting with Rv2027cGene nameConfidence Score
Rv3133cdevR0.963
Rv3133cdevR0.963
Rv0195Rv01950.945
Potential ligand/drug binding sites
No binding sites identified
Similarity to known drug target from sensitive sequence analysis
No drug targets
List of small molecules tested from TIBLE
Data not available
Off-target activity - ligand based from TIBLE
Data not available
Small molecules involved in protein-protein complex from TIMBAL .Proteins similar to Mtb based on sequence analysis.
Data not available
STITCH interactions  
Chemical NameConfidence scoreMolecular Weight
hydride acetatePubChem0.961.0599
molecular oxygenPubChem0.931.9988
acetatePubChem0.959.044